Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform

Figure 2 Biomarker proteins with different expression level in WT-cagA, phosphorylation site mutant cagA (MT-cagA) and blank-vector-transfected AGS cells detected by ProteinChip arrays. Cell lysates of each group were analyzed on SAX2 and CM10 Chip surfaces under laser intensity of 180 and sensitivity of 7. One representative spectrum was selected from a quadruplicate set of samples for each group. The top panel in each figure is the spectral view, and the lower panel is the gel view. Peaks with different expression level were marked with m/z values. A: Biomarkers detected by SAX2; B: Biomarkers detected by CM10. ¹Indicates CagA tyrosine-phosphorylation-independent proteins, which can be induced by both WT CagA and MT CagA (adopted from Chinese J Cell Biology 2006; 28: 603-610).