Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform

Figure 1 Detection of phosphorylated ERK1/2 in transfected AGS cells. Cells transfected with WT-cagA or blank vectors were stimulated with serum by adding fresh medium containing 10% FCS for 0, 1 and 3 h, after 24 h serum starvation, and then harvested for Western blot analysis of total and phosphorylated ERK1/2 (P-ERK1/2). The level of P-ERK1/2 in WT-cagA transfectants was significantly higher than that in blank vector transfectants and untransfected AGS cells at all three time points of serum stimulation. No obvious increases in total ERK among the three groups were found. GAPDH level was used as a loading control.