Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform

Table 3 Swiss-Prot and TrEMBL database searching results of the three ERK related biomarkers

Biomarker	Meaningful matching information in Swiss-Prot and TrEMBL database			Comments
(Mw)	Protein Name	Mw	pI
9084 (SAX2)	BIM-alpha3 (BCL2-like 11 transcript variant 10, chain 1-79)	9081	5.42	Over-expression of BIM-alpha3 results in cell apoptosis
	EP2E (chain 1-80)	9091	7.55	Part of the protein sequences homologous to β-defensins, a family of antimicrobial peptides.
	UPF0197 protein (C11orf10 chain 1-79)	9079	5.58	Expressed in CD34+ hematopoietic stem/progenitor cells
4229 (CM10)	Chromogranin-A Precursor (ER-37, chain 420-456)	4233	4.59	Neuroendocrine and endocrine secretory granules, considered as biomarkers in some human cancers
8162 (CM10)	Small-inducible cytokine B6 Precursor (chain 40-114)	8162	9.74	Functional characterization as chemokines, chemotactic for neutrophil granulocytes
	Putative microRNA host gene protein 1 (chain 1-70)	8163	9.25	Highly expressed in B-cell lymphoma and lung cancer and enhanced cell proliferation
	PNAS-145 (Chain: 1-68)	8161	11.24	Human acute promyelocytic leukemia cell line NB4’s apoptosis/differentiation related genes

Searching was carried out using TagIdent tool with mass and the pI information (0-9.0 for SAX2, 4.0-14.0 for CM10).