Decreased phagocytic activity of Kupffer cells in a rat nonalcoholic steatohepatitis model

Figure 2 Histological analysis of the liver sections. A: Hematoxylin and eosin stain (× 200); B: Azan stain (× 100); C: Sirius red stain (× 100). Histological examination of the liver tissue of 1-wk CDAA-fed rats showed inflammation and fat deposits, but no fibrosis, corresponding to Matteoni’s type 2. The 4-wk CDAA-fed rats had more inflammation, fat deposits, and fibrosis, which was equivalent to Matteoni’s type 3 and grade 2/stage 2 of Brunt’s NASH classification. The histological findings in the 8-wk CDAA-fed rats were equivalent to Matteoni’s type 4 and Brunt’s grade 2/stage 3. In the 4 and 8-wk groups, Sirius red staining revealed abundant collagen (n = 5).