Fibrinogen-like protein 2/fibroleukin prothrombinase contributes to tumor hypercoagulability via IL-2 and IFN-γ

Figure 5 Increased expression of fgl2 after IFN-γ or IL-2 stimulation. I: HUVEC and THP-1 cells were treated with IFN-γ (200 U/mL) and IL-2 (100 U/mL) and stained with antibodies specific for hfgl2. A: IFN-γ (200 U/mL, 12 h) + HUVEC (SP, × 200); B: IL-2 (100 U/mL, 8 h) + HUVEC (SP, × 200); C: NS +HUVEC (SP, × 200); D: IFN-γ (200 U/mL, 12 h) + THP-1 (SP, × 200); E: IL-2 (100 U/mL, 8 h) + THP-1(SP, × 200); F: NS + THP-1 (SP, × 200). II: Western blotting analysis of hfgl2 expression after IFN-γ (200 U/mL) and IL-2 (100 U/mL) stimulation for 12 h and 8 h on HUVEC and THP-1 cells. 1: Intact HUVEC; 2: IFN-γ + HUVEC; 3: Intact THP-1; 4: IFN-γ + THP-1; 5: IL-2 + HUVEC; 6: IL-2 +THP-1. III: Real-time PCR analysis of inducible hfgl2 mRNA after stimulation for 4 h. ^aP = 0.0329, vs HUVEC alone; ^bP = 0.0059, vs THP-1 alone.