Fibrinogen-like protein 2/fibroleukin prothrombinase contributes to tumor hypercoagulability via IL-2 and IFN-γ

Figure 4 Fgl2 expression evidenced in mouse tumor tissue. Male BALB/c-nu/nu mice were subcutaneously injected MHCC97LM6 cell lines and tumor tissues were harvested 36 d later. Panel I: A-C, HE staining in tumor tissue at injection site (A, × 200; B, × 400) and lung metastatic tumor tissue (C, × 200); D-I: mfgl2 expression in tumor tissue of human hepatocellular carcinoma (HCC) nude mice model (D, SP × 200; E, SP × 400; G, dual staining of mfgl2 and marker of macrophages; H, dual staining of mfgl2 and marker of endothelial cells; F and I, negative controls). Arrows in D and E indicate fgl2 positive cells, arrows in G and F indicate the fgl2 positive macrophage and endothelial cells, respectively. Panel II: mfgl2 mRNA expression in tumor tissue (1), PCDNA3.1-fgl2 plasmid as positive control (2), PCDNA3.1 as negative control (3) and DL-2000 marker (4).