Distinct expression patterns in hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma

Table 2 Primers used to amplify cDNA at various genes in real time RT-PCR

Target gene	Objective	Forward primer sequence (5'-3')	Reverse primer sequence (3'-5')	Genebank accession no./Amplicon size
RPS5	qRT-PCR	GTATGCCGCCAAACGCTTC	CGCCTGTGAGCAGGTGTAT	NM_001009, 152 bp
KRT8	qRT-PCR	GGAGGCATCACCGCAGTTAC	GGTTGGCAATATCCTCGTACTGT	NM_002273, 637 bp
CFLAR	qRT-PCR	GACAGAGCTTCTTCGAGACAC	GCTCGGGCATACAGGCAAAT	AF009616, 116 bp
ATP5F1	qRT-PCR	ACTGGGCTTATCTTGTACGCT	GCAAAGTCTGCAACAAAGGGA	NM_001688, 131 bp
IGFBP2	qRT-PCR	GACAATGGCGATGACCACTCA	GCTCCTTCATACCCGACTTGA	NM_000597, 121 bp
MAP3K5	qRT-PCR	AAAAAGGCATTTGAATCTGAGCC	GCTTGAATGACTCTCATGTGGTC	NM_005923, 233 bp
MMP9	qRT-PCR	GGGACGCAGACATCGTCATC	TCGTCATCGTCGAAATGGGC	NM_004994, 139 bp
VIM	qRT-PCR	AGTCCACTGAGTACCGGAGAC	CATTTCACGCATCTGGCGTTC	AK093924, 98 bp
ACTB	qRT-PCR	CATGTACGTTGCTATCCAGGC	CTCCTTAATGTCACGCACGAT	NM_001101, 250 bp
GAPD	qRT-PCR	CAACTGGTCGTGGACAACCAT	GCACGGACACTCACAATGTTC	AC002389, 260 bp
CD58	qRT-PCR	CTCATGGGATTGTCCTATGGAGC	GCTTGGGATACAGGTTGTCAAA	NM_001779, 154 bp