Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Oct 21, 2008; 14(39): 6072-6077
Published online Oct 21, 2008. doi: 10.3748/wjg.14.6072
Published online Oct 21, 2008. doi: 10.3748/wjg.14.6072
Table 2 Primers used to amplify cDNA at various genes in real time RT-PCR
Target gene | Objective | Forward primer sequence (5'-3') | Reverse primer sequence (3'-5') | Genebank accession no./Amplicon size |
RPS5 | qRT-PCR | GTATGCCGCCAAACGCTTC | CGCCTGTGAGCAGGTGTAT | NM_001009, 152 bp |
KRT8 | qRT-PCR | GGAGGCATCACCGCAGTTAC | GGTTGGCAATATCCTCGTACTGT | NM_002273, 637 bp |
CFLAR | qRT-PCR | GACAGAGCTTCTTCGAGACAC | GCTCGGGCATACAGGCAAAT | AF009616, 116 bp |
ATP5F1 | qRT-PCR | ACTGGGCTTATCTTGTACGCT | GCAAAGTCTGCAACAAAGGGA | NM_001688, 131 bp |
IGFBP2 | qRT-PCR | GACAATGGCGATGACCACTCA | GCTCCTTCATACCCGACTTGA | NM_000597, 121 bp |
MAP3K5 | qRT-PCR | AAAAAGGCATTTGAATCTGAGCC | GCTTGAATGACTCTCATGTGGTC | NM_005923, 233 bp |
MMP9 | qRT-PCR | GGGACGCAGACATCGTCATC | TCGTCATCGTCGAAATGGGC | NM_004994, 139 bp |
VIM | qRT-PCR | AGTCCACTGAGTACCGGAGAC | CATTTCACGCATCTGGCGTTC | AK093924, 98 bp |
ACTB | qRT-PCR | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | NM_001101, 250 bp |
GAPD | qRT-PCR | CAACTGGTCGTGGACAACCAT | GCACGGACACTCACAATGTTC | AC002389, 260 bp |
CD58 | qRT-PCR | CTCATGGGATTGTCCTATGGAGC | GCTTGGGATACAGGTTGTCAAA | NM_001779, 154 bp |
- Citation: Lee CF, Ling ZQ, Zhao T, Lee KR. Distinct expression patterns in hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma. World J Gastroenterol 2008; 14(39): 6072-6077
- URL: https://www.wjgnet.com/1007-9327/full/v14/i39/6072.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.6072