Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

Figure 1 Visualization of donor-derived cells in liver sections of S. mansoni-infected mice. Mice chronically infected with S.mansoni were treated with GFP⁺ BMC by injection into the left hepatic lobe (A-F) or by iv route (G and H) and sacrificed after different time points for evaluation by fluorescence microscopy. For visualization of GFP⁺ cells (green), sections were mounted with the nuclear counterstaining with DAPI (blue). A and B: Sections of injected lobe of mice sacrificed 2 h after transplantation; C: Magnification of square area of image B. Sections of livers obtained from mice sacrificed 24 h (D) or 5 d (E) after intralobular BMC injection. F: Magnification of square area of image E; G: GFP⁺ albumin⁺ cells found in liver sections 2 mo after iv injection of BMC; H: Detection of Y chromosome (arrow) in liver section of BMC-treated mice 1 mo after transplantation by iv route.