Depletion of CD4+CD25+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma

Figure 7 CD4⁺CD25⁺ T cells express high levels of Foxp3 transcripts in RLNs and PLNs of CD4⁺CD25^- (P2) and CD4⁺CD25⁺ (P3) T cells (A) which were sorted and stained with FITC-anti-CD25 and PE-anti-CD4 mAbs, and of Foxp3, IL-10, TGF-β1, and β-actin mRNA in CD4⁺CD25⁺ and CD4⁺CD25^- cells of RLNs (B) and PLNs (C) in BaP-only-treated and control mice at wk 32 using RT-PCR (Lanes 1 and 2: BaP-only-treated mice; Lanes 3 and 4: control mice; Lanes 1 and 3: CD4⁺CD25^- cells; Lanes 2 and 4: CD4⁺CD25⁺ cells), as well as of relative Foxp3 from CD4⁺CD25^- and CD4⁺CD25⁺ T cells using real-time quantitative PCR after normalization to HPRT expression (D). ^aP < 0.05 vs control mice.