Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury

Table 2 Reacting system of PCR

Component	Amount
cDNA	4.00 μL
10 × PCR buffer solution	1.96 μL
MgCl2 (25 mmol/L)	2.40 μL
Upper primer (20 pmol/L)	1.00 μL
Down primer (20 pmol/L)	1.00 μL
dNTP (10 mmol/L)	0.36 μL
Taq DNA polymerase (5 IU/μL)	0.10 μL
Deionized water	10.08 μL
Reacting volume	20.00 μL