Expression of Livin and vascular endothelial growth factor in different clinical stages of human esophageal carcinoma

Figure 2 Assay of level of VEGF. A: The expression of VEGF by Western blotting showed that expression of VEGF in clinicopathologic stage two, three and four was significantly higher than that of stage one (^bP < 0.01). Optical density value in clinicopathologic stage two, three and four was significantly higher than that of stage one (^bP < 0.01). Optical density value and positive cell percentage in clinicopathologic stage four was significantly higher than that of stage two and three (^dP < 0.01); B: Up-regulation of VEGF gene transcription matched with the protein level of VEGF that was significantly increased along with the progression of esophageal carcinoma. Optical density value in clinicopathologic stage two, three and four was significantly higher than that of stage one (^fP < 0.01). Optical density value and positive cell percentage in clinicopathologic stage four was significantly higher than that of stage two and three (^hP < 0.01).