Expression of Livin and vascular endothelial growth factor in different clinical stages of human esophageal carcinoma

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Oct 7, 2008 (publication date) through Nov 7, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 7, 2008; 14(37): 5749-5754
Published online Oct 7, 2008. doi: 10.3748/wjg.14.5749

Table 1 Oligonucleotides used for reverse transcriptase-polymerase chain reaction

Target gene	Primer sequence (5’-3’)	Size (bp)	Annealing temperature (°C)
β-actin	Forward: GTTCGCCATGGATGACGATATC	266	59
	Reverse: GCCAGATCTTCTCCATGTCGTC
VEGF	Forward: TGCTCAGCATTCGGACTGACCT	228	61
	Reverse: CAGTATGCATGGACCATGACGG
Livin	Forward: CTGGTCAGAGCCAGTGTTCCT	312	61
	Reverse: TCATAGAAGGAGGCCAGACG

Primers were designed using the Primer Express Program and offered by AuGCT Biotechnology, Beijing, China.

Citation: Chen L, Ren GS, Li F, Sun SQ. Expression of Livin and vascular endothelial growth factor in different clinical stages of human esophageal carcinoma. World J Gastroenterol 2008; 14(37): 5749-5754
URL: https://www.wjgnet.com/1007-9327/full/v14/i37/5749.htm
DOI: https://dx.doi.org/10.3748/wjg.14.5749