Interferon-alpha restrains growth and invasive potential of hepatocellular carcinoma induced by hepatitis B virus X protein

Figure 4 HBx promotes the invasive capacity of Chang liver cells in vitro. A: Chang cells transfected with pcDNA3.1-HBx invaded through the Matrigel were counted on the underside of the transwell filter and compared with Chang/HBx cells (B) and control cells Chang/pcDNA3.1 (C) (× 200). There was no difference between IFN-α-Chang/HBx cells and Chang/pcDNA3.1; D: The average number of migrated cells per site seen under a high-power microscope (× 400) was 41.6 ± 3.1 for the transfected Chang/HBx cells and 7.4 ± 1.2 for IFN-α-Chang/HBx cells and 9.2 ± 1.6 for the control Chang/pcDNA3.1 cells.