Interferon-alpha restrains growth and invasive potential of hepatocellular carcinoma induced by hepatitis B virus X protein

Figure 3 HBx promotes the invasive capacity of Chang liver cells in vitro. Wound-healing assays were performed at 0 and 24 h and observed under a phase-contrast microscope (× 100). A: Chang liver cells with pcDNA3.1-HBx were disrupted, and wound healing occurred after 24 h; B: IFN-α-Chang/HBx cells were disrupted, and the wound did not heal completely after 24 h; C: Chang cells with pcDNA3.1 were disrupted, and the wound did not heal completely after 24 h.