Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line

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Sep 21, 2008 (publication date) through Nov 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 21, 2008; 14(35): 5403-5411
Published online Sep 21, 2008. doi: 10.3748/wjg.14.5403

Table 1 PCR primers

Target genes		Primer sequence	Size (bp)	Annealing temperature (°C)	Cycles
Bcl-2	Sense	5'-GGTGCCACCTGTGGTCCACCT-3'	458	54	35
	Antisense	5'-CCTCACTTGTGGCCCAGATAGG-3'
Bax	Sense	5'-CTGACATGTTTTCTGACGGC-3'	289	54	35
	Antisense	5'-TCAGCCCATCTTCTTCCAGA-3'
Bcl-xl	Sense	5'-TTGGACAATGGACTGGTTG-3'	765	54	35
	Antisense	5'-GTAGAGTGGATGGTCAGTG-3'
Bak	Sense	5'-TGAAAAATGGCTTCGGGGCAAGGC-3'	642	54	35
	Antisense	5'-TCATGATTTGAAGAATCTTCGTACC-3'
GAPDH	Sense	5'-CATGCCAGTGAGCTTCCCGTT-3'	408	56	35
	Antisense	5'-GTGGAGTCTACTGGCGTCTTC-3'
VEGF	Sense	5'-ATGAACTTTCTGCTGTCTTG-3'	382	54	35
	Antisense	5'-TGCATGGTGATGTTGGAC-3'

Citation: Lu YY, Jing DD, Xu M, Wu K, Wang XP. Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line. World J Gastroenterol 2008; 14(35): 5403-5411
URL: https://www.wjgnet.com/1007-9327/full/v14/i35/5403.htm
DOI: https://dx.doi.org/10.3748/wjg.14.5403