Ileal lesions in patients with ulcerative colitis after ileo-rectal anastomosis: Relationship with colonic metaplasia

Figure 3 Endoscopic view, histological analysis and immunohistochemistry against CG3 and Das-1 MoAbs of biopsy samples taken from the neo-terminal ileum and rectal stump from a second UC patient (DLE) with IRA. A-C: Endoscopic views of the neo-terminal ileum at 3 endoscopies, showing focal erosions and ulcers at all times; D: Histological analysis of ileal biopsy samples, compatible with changes towards colonic epithelium, including villous atrophy; E: Immunostochemistry showing staining against CG3 (E1) and Das-1 (E2) MoAbs in the same ileal samples. F-H: Endoscopic view (F), histological (G1, G2) and immunohistochemical analysis against CG3(H1) and Das-1 MoAbs (H2) of the uninvolved ileum, showing no inflammation or colonic metaplasia. I-M: Endoscopic view (I), histological (L1, L2) and immunohistochemical analysis using CG3(M1) and Das-1 MoAbs (M2) of the rectal stump, showing typical UC lesions and staining against hTM5-related antigens.