Phosphatidylinositol 3-kinase/Akt pathway regulates hepatic stellate cell apoptosis

Figure 2 Immunocytochemistry (× 400). A: In negative control, the primary antibody was omitted; B and E: PI 3-K p85 and p-Akt⁴⁷³ staining in the PDGF-BB group; C and F: PI 3-K p85 and p-Akt473 expression in the PDGF-BB and LY 294002 groups; D and G: PI 3-K p85 and p-Akt⁴⁷³ staining in the LY 294002 group. Rabbit anti-PI 3-K p85α polyclonal antibody (1:50) and rabbit anti-phospho-Ser⁴⁷³ Akt polyclonal antibody (1:100) used as primary antibodies.