Mucosal cytokine network in inflammatory bowel disease

Table 1 IL-17A-induced genes in human colonic myofibroblasts

Gene name	Ref seq ID.	Fold increase
CXCL1 (Gro-α)	NM_001511.1	× 26.11
IL-20 receptor, alpha	NM_014432.1	× 15.22
CCR5	NM_000579.1	× 12.75
CXCL6 (GCP-2)	NM_002993.1	× 12.45
CXCL3 (Gro-γ)	NM_002090.1	× 11.70
MMP-16	NM_005941.2	× 10.41
CXCL2 (Gro-β)	NM_002089.1	× 9.75
IRAK3	NM_007199.1	× 9.06
IL-8	NM_000584.2	× 8.91
IL-22 receptor 1	NM_021258.1	× 8.22
MMP-1	NM_004142.1	× 6.35
Complement component 3	NM_000064.1	× 5.56
IL-6	NM_000600.1	× 4.63
Carbonic anhydrase XII	NM_001218.1	× 4.42
Superoxide dismutase 2	NM_000636.1	× 4.25
CCL19 (MIP-3b)	NM_006274.2	× 3.74
CCL7 (MCP-3)	NM_006273.2	× 2.77
CCL2 (MCP-1)	NM_002982.2	× 2.24

Human colonic subepithelial myofibroblasts were stimulated with IL-17A (200 ng/mL) for 12 h, and the changes in gene expression were assessed by IntelliGene HS Human Expression Chip (Takara-Bio, Kyoto, Japan). The fold change values were determined as a ratio of Cy5 signal intensity (IL-17A stimulated values)/Cy3 signal intensity (non-stimulated values). The data were average of three independent analysis. The NCBI reference sequence code was presented following the gene name.