Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma

Figure 3 Representative results of Western blot in human GC tissue samples. β-tubulin was used as an internal control. CHFR protein expression level in GS tissue samples was significantly lower than that in paired normal gastric mucosa samples. Aberrant methylation of CHFR and down-regulation or loss of CHFR mRNA expression were detected in 16 poorly differentiated adenocarcinoma tissue samples and 1 mucinous adenocarcinoma tissue sample, while positive protein expression of CHFR was detected in 9 moderately-differentiated adenocarcinoma tissue samples without CHFR methylation. N: Normal gastric mucosa corresponding to tumors; T: Gastric cancer.