OCTN and CARD15 gene polymorphism in Chinese patients with inflammatory bowel disease

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Aug 21, 2008 (publication date) through Jul 3, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 21, 2008; 14(31): 4923-4927
Published online Aug 21, 2008. doi: 10.3748/wjg.14.4923

Table 2 Primer sequences and restriction enzymes used for RFLP genotyping

SNP	Primers	Restriction enzyme	Length of restriction fragments
OCTN1 1672C/T	F: CGTCATGGGTAGTCTGACTGTCCTGATTGGGATC	BamH I	Allel C: 30 + 88 bp
(rs1050152)	R: TCCTACTTACCATTTCACTTTCTGCATCTGCTCTAAGG	BamH I	Allel T: 118 bp
OCTN2 -207G/C	F: GCGCCGCTCTGCCTGCCAG	Msp I	Allel G: 44 + 83 bp
(rs2631367)	R: AGGGTAGGCTCGCGAGCTGACACC	Msp I	Allel C: 127 bp
R702W 2104C/T	F: TGGGGCCTGCTGGCTGAGTG	Msp I	Allel C: 76 + 45 bp
(rs2066844)	R: GTGCAGCTGGCGGGATGGAG	Msp I	Allel T: 121 bp
G908R 2722G/C	F: TCTGGCTGGGACTGCAGAGG	BstU I	Allel G: 131 bp
(rs2066845)	R: CCCCTCGTCACCCACTCTGTCGC	BstU I	Allel C: 109 + 22 bp

Citation: Li M, Gao X, Guo CC, Wu KC, Zhang X, Hu PJ. OCTN and CARD15 gene polymorphism in Chinese patients with inflammatory bowel disease. World J Gastroenterol 2008; 14(31): 4923-4927
URL: https://www.wjgnet.com/1007-9327/full/v14/i31/4923.htm
DOI: https://dx.doi.org/10.3748/wjg.14.4923