Rapid Communication
Copyright ©2008 The WJG Press and Baishideng.
World J Gastroenterol. Aug 14, 2008; 14(30): 4816-4822
Published online Aug 14, 2008. doi: 10.3748/wjg.14.4816
Table 1 Major information relative to PCR for amplifying 7 genes of H pylori
Target genesPrimer sequences (5’-3’)ReferenceTm (°C)Products (bp)
ureBF: CGTCCGGCAATAGCTGCCATAGT[18]55463
R: GTAGGTCCTGCTACTGAAGCCTTA
hpaAF: CTTTTAGGTGCGAGCGTGGTG[19]54716
R: AATTCCTTGGCGTCTTTTTGATAA
vacAF: ATGGAAATACAACAAACACACCG[20]54337
R: CAACCTCCATCAATCTTACTGGA
CagAF: ATAAGTCTAAATTAGACAACTTGAGCGA[21]56297
R: TTAGAATAATCAACAAAC ATCACGCCAT
napAF: TGCGATCGTGTTGTTTATG[22]50344
R: GATCGTCCGCATAAGTTAC
flaAF: GCTTTTTCAGGTCAATAC[13]52524
R: GTAGCGATACGAACCTGA
flaBF: GATAAATACCAATATCGC[13]52241
R: CTCATCCATCGCTTTATC