Inhibition of hepatitis B virus gene expression and replication by artificial microRNA

Figure 4 A: Effect of amiRNA-HBV with transient transfection on HBV DNA levels; B: Effect of amiRNA-HBV with stable transfection on HBV DNA levels, The amounts of HBV DNA are expressed as mean ± SD. Compared with negative control, all three plasmids had significant inhibitory effect on HBsAg and HBeAg at 48 h, 72 h and 96 h after transfection (P = 0.049, 0.000021 and 0.0011 for S89 group; P = 0.0002, 0.000016 and 0.0012 for S367 group; P = 0.0003, 0.00006 and 0.00016 for S608 group). All three plasmids had significant inhibitory effect on HBV DNA in stably transfected groups, (P = 0.00009, 0.000007 and 0.000006, respectively).