Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocellular carcinoma

Figure 6 After HepG2 cells were transfected with LV-shMAT2B for 72 h, western blot was used to detect the protein levels of cyclin D1 and cyclin D2, bcl-xL and bcl-xs, results show the expressions of cyclin D1 (B)and Bcl-xL (C) were decreased compared to controls and untreated cells. The expression of bcl-xs (D) was increased but that of cyclin D2 did not change (A). The expression of MAT2B was suppressed by LV-shMAT2B significantly (E). U, untreated HepG2 cell; S, HepG2 cell treated with scrambled siRNA; LV, HepG2 cells treated with LV-shMAT2B. The protein level of MAT2B was measured by western blot.