Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocellular carcinoma

Figure 2 MAT2B gene was cloned from the cDNA library with production of 1020 bp by PCR (B), pEGFP-C1-MAT2B was constructed after restricted enzyme cutting and connection with production of 420 bp by PCR as showed in Figure2C. Plasmids containing siMAT2B (pGCL-GFP-siMAT2B) were co-transfected with pEGFP-C1-MAT2B in 293T cell, phase contrast and GFP expression under a fluorescent microscope was taken after 72 h (A). Protein level of MAT2B in 293T cells was detected by western blot (D). pGCL-GFP-siMAT2B-1 and -4 can significantly knock down expression of MAT2B at the protein level (D). PC, pcDNA3.1; NC, non-silence control vector. Lanes 1-4: pGCL-GFP-siMAT2B-1, -2, -3 and -4, respectively.