Single nucleotide polymorphism in the tumor necrosis factor-alpha gene affects inflammatory bowel diseases risk

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Aug 7, 2008 (publication date) through Nov 4, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Clinical Research

World J Gastroenterol. Aug 7, 2008; 14(29): 4652-4661
Published online Aug 7, 2008. doi: 10.3748/wjg.14.4652

Table 2 Primer and probe sequences for custom made TaqMan SNP genotyping assay for TNF-α -857C/T, TNF-α -308G/A and TNF-α -238G/A

TaqMan SNP genotyping assay	DNA sequence
TNF-α_-857C/T _forward primer	5’-GGGCTATGGAAGTCGAGTATGG -3’
TNF-α_-857C/T _reverse primer	5’-GTCCTGGAGGCTCTTTCACT -3’
TNF-α_-857C/T_VIC probe	5’-CCCTGTCTTCGTTAAGG -3’
TNF-α_-857C/T _FAM probe	5’-CCTGTCTTCATTAAGG -3’
TNF-α_-308G/A_forward primer	5’-CCAAAAGAAATGGAGGCAATAGGTT -3’
TNF-α_-308G/A _reverse primer	5’-GGACCCTGGAGGCTGAAC -3’
TNF-α_-308G/A _VIC probe	5’-CCCGTCCCCATGCC -3’
TNF-α_-308G/A _FAM probe	5’-CCCGTCCTCATGCC -3’
TNF-α_-238G/A_forward primer	5’-CAGTCAGTGGCCCAGAAGAC -3’
TNF-α_-238G/A _reverse primer	5’-CCCTCACACTCCCCATCCT -3’
TNF-α_-238G/A _VIC probe	5’-CTCGGAATCGGAGCAG -3’
TNF-α_-238G/A _FAM probe	5’-CTCGGAATCAGAGCAG -3’

Citation: Ferguson LR, Huebner C, Petermann I, Gearry RB, Barclay ML, Demmers P, McCulloch A, Han DY. Single nucleotide polymorphism in the tumor necrosis factor-alpha gene affects inflammatory bowel diseases risk. World J Gastroenterol 2008; 14(29): 4652-4661
URL: https://www.wjgnet.com/1007-9327/full/v14/i29/4652.htm
DOI: https://dx.doi.org/10.3748/wjg.14.4652