Lysophosphatidic acid induced nuclear translocation of nuclear factor-κB in Panc-1 cells by mobilizing cytosolic free calcium

Figure 5 Localization of NF-κB p65 in Panc-1 cells. A: PTX-treatment prevented nuclear translocation of NF-κB p65 induced by LPA in Panc-1 cells at 1 h. Panc-1 cells were preincubated with 100 ng/mL PTX, and then stimulated with 10 &mgr;mol/L LPA; B: U73122-treatment prevented nuclear translocation of NF-κB p65 induced by LPA in Panc-1 cells at 1 h. Panc-1 cells were pretreated with 10 &mgr;mol/L U73122 for 10 min before adding 10 &mgr;mol/L LPA; C: Nuclear translocation of p65 induced by LPA was reduced by an intracellular calcium chelator, BPTA-AM at 1 h. Panc-1 cells were treated with 10 &mgr;mol/L BAPTA-AM for 15 min and then stimulated with 10 &mgr;mol/L LPA; D: Thapsigargin alone caused nuclear translocation of p65 at 1 h. Panc-1 cells were treated with 500 nmol/L thapsigarigin; E: Phorbol myristate (PMA) alone failed to mobilize p65 into nuclei at 1 h. Panc-1 cells were treated with 100 nmol/L PMA; F: Staurosporine reduced nuclear localization of p65 induced by LPA at 1 h. Panc-1 cells were treated with 10 nmol/L staurosporine for 10 min and then stimulated with 10 &mgr;mol/L LPA.