Lysophosphatidic acid induced nuclear translocation of nuclear factor-κB in Panc-1 cells by mobilizing cytosolic free calcium

Figure 4 Effect of LPA on nuclear translocation of NF-κB. LPA-induced nuclear translocation of p65 in Panc-1 cells. Localization of p65 was visualized by indirect immunofluorescence staining using rabbit anti-p65 polyclonal antibodies (1:50) which only recognized NF-κB p65. Donkey anti-rabbit antibodies (1:100) conjugated to rhodamine was performed, and visualized under a confocal microscope. A: Cytosolic localization of p65 in an inactive form in unstimulated Panc-1 cells; B: Nuclear localization of p65 in Panc-1 cells after treatment of 10 &mgr;mol/L LPA was observed at 60 min.