Lysophosphatidic acid induced nuclear translocation of nuclear factor-κB in Panc-1 cells by mobilizing cytosolic free calcium

Figure 2 Effect of LPA on cytosolic free calcium concentration [Ca²⁺]_i in Panc-1 cells. A: Representative Ca²⁺ signal was evoked by 10 &mgr;mol/L LPA in Panc-1 cells; B: Effect of chelation of extracellular Ca²⁺ by addition of 0.2 mmol/L EDTA on LPA-induced increases in Ca²⁺; C: PTX-sensitive effect of LPA on cytosolic free calcium. Panc-1 cells were pretreated with 100 ng/mL of PTX for overnight and loaded with fura-2/AM; D: Effect of U73122, a phospholipase C inhibitor, on LPA-induced increases in cytosolic free calcium in Panc-1 cells. Panc-1 cells were treated with U73122 at a concentration of 10 &mgr;mol/L for 10 min, and then stimulated by 10 &mgr;mol/L LPA; E: Effect of U73343, an inactive analogue of U73122, on LPA-evoked increases in cytosolic free calcium in Panc-1 cells.