Association between calcium sensing receptor gene polymorphisms and chronic pancreatitis in a US population: Role of serine protease inhibitor Kazal 1type and alcohol

Table 1 Polymerase chain reaction primer pairs, magnesium concentration and annealing temperatures used for genetic analysis of the CASR gene

Scanning region	Forward and reverse primer sequences	MgCl2 (mmol/L)	Annealing temperature (°C)
Exon 2	5’-ACCACCCACATTACAAGTC-3’	2.5	55
	5’-GCTTTTCTCCAACCACTCAG-3’
Exon 3	5’-ATGAAGCCAGAGAGTAGTAAC-3’	2.5	58
	5’-TAAACCGTATGGCTATTGGG-3’
Exon 4a	5’-GCTTTTCCTTACCCTTTCTTTCATC-3’	2	58
	5’-ATCACCTCTACCACATGCTG-3’
Exon 4b	5’-CAGATCTTGAGCCCCTCATC-3’	2	59
	5’-GCAGCCCAACTCTGCTTTAT-3’
Exon 5	5’-TGGGGCTTGTACTCATTCTT-3’	1.5	59
	5’-CTGGTTTTCTGATGGACAGC-3’
Exon 7a	5’-CACACAATAACTCACTCTTCAC-3’	2.0	61
	5’-CAGAGGAAAACCAGCAGGAAC-3’
Exon 7b	5’-AAAACCAACCGTGTCCTCCTG-3’	1.0	53
	5’-ATGGCAATCACCTCTACGGC-3’
Exon 7c	5’-GCTCATCTTCTTCATCGTCTGG-3’	1.0	58
	5’-CGTATCGCTGCTTTTCTGGG-3’
Exon 7d	5’-CCCAGCAAGAGCAGCAG-3’	1.0	58
	5’-ACAACTCTTCAGGGTCCTCC-3’