Clinical significance of NOD2/CARD15 and Toll-like receptor 4 gene single nucleotide polymorphisms in inflammatory bowel disease

Table 1 Primers sequences and restriction enzymes used for genotyping TLR4 and NOD2/CARD15

Gene locus	SNPs		Sequence	Restriction enzyme
NOD2/CARD15	R702W	For	5’ TTCAGATCACAGCAGCCTTC 3’	MspI
		Rev	5’ CCCACACTGCAAAATGTCAAC 3’
	G908R	For	5’ AGCCACTGAAAACTCTTGG 3’	HhaI
		Rev	5’ TCTTCACCTGATCTCCCCAA 3’
	L1007finsC	For	5’ CCTGCAGTCTCTTTAACTGG 3’	NlaIV
		Rev	5’ CTTACCAGACTTCCAGGATG 3’
TLR4	D299G	For	5’ TTAGAAATGAAGGAAACTTGGAAAAG 3’	BsaBI
		Rev	5’ TTTGTCAAACAATTAAATAAGTGATTAATA 3’
	T399I	For	5’ GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA 3’	HinfI
		Rev	5’ CCTGAAGACTGGAGAGTGAGTTAAATGCT 3’