Effects of ethanol on insulin-like growth factor-I system in primary cultured rat hepatocytes: Implications of JNK1/2 and alcoholdehydrogenase

Figure 4 Effects of ADH inhibitor on the IGF-I system, cell viability, and JNK1/2 activity induced by ethanol at 180 min in primary cultured rat hepatocytes (mean ± SD). The cells were pretreated with 200 &mgr;mol/L 4-MP 30 min before being exposed to 200 mmol/L ethanol for 180 min. A: p-JNK1/2, t-JNK1/2, and IGF-IR activities; B: IGF-I concentration; C and D: IGF-I mRNA expression; E: Cell viability. β-actin (A) and GAPDH (C) were used as loading controls. The mRNA expression (as indicated by a band at 180 bp, C) was determined by densitometric analysis (D) of the amplification products. Data represent percentages relative to control. The cell viability (D) was determined by the MTT assay. ^aP < 0.05 vs control (n = 6).