Effects of ethanol on insulin-like growth factor-I system in primary cultured rat hepatocytes: Implications of JNK1/2 and alcoholdehydrogenase

Figure 1 Time course of effects on the IGF-I system, JNK1/2 activity, and cell viability induced by ethanol in primary cultured rat hepatocytes (mean ± SD). The cells were exposed to 200 mmol/L ethanol for 0, 60, 120 and 180 min. A: P-JNK1/2, t-JNK1/2, and IGF-IR activities; B: IGF-I concentration; C and D: IGF-I mRNA expression; E: Cell viability. β-actin (A) and GAPDH (C) were used as loading controls. The mRNA expression (as indicated by a band at 180 bp, C) was determined by densitometric analysis (D) of the amplification products. Data represent percentages relative to control. The cell viability (D) was determined by the MTT assay. ^aP < 0.05, ^bP < 0.01 vs control (n = 6).