Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells

Figure 7 Protein expression levels of active caspase-3 and cleaved PARP in CWO-induced apoptosis. HepG2 cells were treated with medium alone (control) or different concentration of CWO for 48 h. Cells of each sample were counted to 1.0 × 10⁶ and all the samples were normalized to final protein concentration in 0.2 &mgr;g/&mgr;L. It was detected with BD^TM CBA Human Apoptosis Kit (BD, Franklin Lakes, USA) according to manufacturer instruction. The results were analyzed by FCAP Array V1.0. Active caspase-3 protein level in HepG2 was shown in (A) and the cleaved PARP protein level in HepG2 was shown in (B). The x-axis indicated the concentration of CWO while the y-axis indicated amount of proteins (unit per mL). Concentration of active caspase-3 and cleaved PARP in test samples were determined using the standard curve. Data expressed as mean ± SD from three independent experiments. ^bP < 0.01, ^dP < 0.001 vs control.