Bcl-xL and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells

Figure 2 Drug-induced apoptosis in CRC cells. SW480 cells were treated with the chemotherapeutic agents 5-FU, oxalipatin (“oxali”) and irinotecan (“irino”) for 48 h (concentrations as indicated, upper panel). In addition, cells were treated with the EGFR1 antibody cetuximab (“cetux”, 20 &mgr;g/mL) or the EGFR1 tyrosine kinase inhibitor PD168393 (7 &mgr;mol/L, lower panel) in FCS reduced medium. Cells were then harvested and analyzed for apoptosis induction by flow cytometry. Assays were performed in triplicates. Values are mean ± SD.