Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Jun 28, 2008; 14(24): 3804-3811
Published online Jun 28, 2008. doi: 10.3748/wjg.14.3804
Published online Jun 28, 2008. doi: 10.3748/wjg.14.3804
Table 1 Primer sequence, length and annealing temperature of exons 5-8 in PTEN gene
| Primer sequence | Primer length (bp) | Amplification fragment length (bp) | Annealing temperature (°C) | |
| Exon-5F: | ACCTGTTAAGTTTGTATGCAAC | 22 | 379 | 52 |
| R | TCCAGGAAGAGGAAAGGAAA | 20 | ||
| Exon-6F: | CATAGCAATTTAGTGAAATAACT | 23 | 274 | 52 |
| R | GATATGGTTAAGAAAACTGTTC | 22 | ||
| Exon-7F: | TGACAGTTTGACAGTTAAAGG | 21 | 263 | 58 |
| R | GGATATTTCTCCCAATGAAAG | 21 | ||
| Exon-8F: | CTCAGATTGCCTTATAATAGTC | 22 | 558 | 52 |
| R | TCTGTTACTTGCTACGTAAAC | 21 |
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Citation: Guo CY, Xu XF, Wu JY, Liu SF. PCR-SSCP-DNA sequencing method in detecting
PTE N gene mutation and its significance in human gastric cancer. World J Gastroenterol 2008; 14(24): 3804-3811 - URL: https://www.wjgnet.com/1007-9327/full/v14/i24/3804.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.3804