Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 17 In vitro effect of NG and silymarin on free radicals generation. Free radicals scavenging effect of NG and silymarin was studied against DPPH radicals (stable hydroxyl radical), enzymic, non-enzymic (superoxide radicals) and ABTS radical (stable hydroxyl radical). Percentage anti-oxidant activity (% effect) was determined as described in Materials and Methods section. Values are mean from five independent determinations. ^bP < 0.001 versus control. Control O.D. system DPPH, 0.650, system Enzymatic, 0.250 and system Non-enzymatic, 0.300 and system ABTS, 0.750. Data are expressed as mean ± SD and are from a representative experiments repeated twice and conducted in triplicate. Statistical significance: ^aP < 0.05; ^bP < 0.01; ^cP < 0.02; ^dP < 0.001; NS = non-significant vs respective controls.