Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 12 Effect of NG and silymarin against CCl₄-induced DNA fragmentation. HuH-7 cells were pre-incubated for 1 h with test materials (NG and silymarin) at mentioned concentrations. The cells were further incubated for 24 h with CCl₄ (2 mmol/L). Thereafter, genomic DNA was extracted from cells and subjected to gel electrophoresis as mentioned in Materials and Methods section. Lanes: 1: Control; 2: CCl₄ 2 mmol/L; 3: CCl₄ 2 mmol/L + NG 30 mg/L; 4: CCl₄ 2 mmol/L + NG 100 mg/L; 5: CCl₄ 2 mmol/L+ Silymarin 50 mg/L; 6: Ladder.