Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 10 Effect of NG and silymarin against CCl₄-induced cytochrome C release from isolated rat liver mitochondria. CCl₄ treatment causes cytochrome C release form rat liver mitochondria. Mitochondria were treated with CCl₄ in presence or absence of test materials (NG and silymarin) and cytochrome C levels were measured as described in Materials and Methods section. Data are expressed as mean ± SD and are from a representative experiments repeated twice and conducted in triplicate. Statistical significance: ^bP < 0.001 vs the untreated control; ^aP < 0.02; ^dP < 0.001 and NS = non-significant vs CCl₄ treated mitochondria.