Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 9 Effect of NG and silymarin against caspase 3-mediated apoptosis. CCl₄ treatment increases caspase 3 activity in HuH-7 cells. HuH-7 cells were treated with CCl₄ in presence or absence of test materials (NG and silymarin) and caspase 3 activity was measured as described in Materials and Methods section. Data are expressed as mean ± SD and are from representative experiments repeated twice and conducted in triplicate. Statistical significance: ^bP < 0.001 vs the untreated control. ^dP < 0.01; ^fP < 0.001 vs CCl₄-treated cells.