Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 7 Effect of NG and silymarin on CCl₄-induced ROS production. HuH-7 cells were pre-incubated for 1 h with medium containing test materials (NG and silymarin) at different concentrations (10 to 100 mg/L). The cells were kept for further incubation in absence or presence of 2 mmol/L CCl₄ (stimulated). After 24 h of incubation maintaining the specific treatments, the cells were incubated with serum-free medium containing DCF-DA and 123-DHR and ROS levels were studied as mentioned in material and methods section. H₂O₂ (100 &mgr;mol/L) was used as positive control for ROS generation. Data are expressed as mean ± SD and are from representative experiments repeated twice and conducted in triplicate. Statistical significance, ^bP < 0.001 vs the untreated control; ^dP < 0.001, ^aP < 0.05; ^fP < 0.01 vs CCl₄-treated cells.