Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 5 Effect of NG and silymarin in vitro on CYP2E1 catalytic activity. A: Rat liver microsomes were incubated in the absence or presence of different concentrations (5 to 100 mg/L) of test materials. CYP2E1 activity was assayed by following the hydroxylation of aniline hydrochloride in presence of cumene hydroperoxide as described in Materials and Methods section; B: Concentration dependent protection by NG and silymarin against alcohol (2.5 mmol/L)-induced aniline hydroxylase levels in rat liver microsomes. The microsomes were pre-incubated with medium containing various concentrations (5 to 100 mg/L) of test materials for 5 min before addition of alcohol and aniline hydroxylase levels were determined. Data are expressed as mean ± SEM and are from a representative experiments repeated twice and conducted in triplicate. P values: ^bP < 0.001 vs the corresponding alcohol-treated microsomes; ^dP < 0.001 and ^fP < 0.001 vs alcohol-treated cells in presence of test materials; NS: Non-significant.