Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 1 Finger print profile and chemical structure of NG. The HPLC profile of NG was performed by employing Shimadzu HPLC system consisting of a diode array detector and C18 column (5 &mgr;m, 250 mm x 4.0 mm I.D.) by UV detection at 254 nm. NG was resolved on a mobile phase consisting of methanol: 2% acetonitrile (30:70) and delivered at a flow rate of 0.6 mL/min. The chromatogram is representative of one of three independent analyses.