Partial overlap of anti-mycobacterial, and anti-Saccharomyces cerevisiae mannan antibodies in Crohn’s disease

Figure 2 Determination of anti-mycobacterial serum IgG in IBD and healthy controls. ELISA for different mycobacterial lysates and purified LAM or ManLAM were performed with sera from 105 CD patients, 45 UC patients and 35 controls. A negative to positive discrimination level was defined by a cut-off value representing the average of all A₄₅₀ values from the healthy controls (excluding the clearly positive individuals with an A₄₅₀≥ 0.3) plus 3 standard deviations. With these cut-off values, individuals positive for antibodies against the various mycobacterial antigens were identified and are shown as a percent of the total population. Asterisks indicate significant differences between the populations. ^aP < 0.05, ^bP < 0.01.