Partial overlap of anti-mycobacterial, and anti-Saccharomyces cerevisiae mannan antibodies in Crohn’s disease

Figure 1 Reactivity of Galanthus nivalis lectin (GNL) with mycobacterial lysates and purified LAM or ManLAM. ELISA plates were coated with yeast mannan, mycobacterial lysates, purified LAM or ManLAM or coating buffer alone without antigen (-), and binding of biotinylated GNL to these antigens assessed by further incubation with peroxidase-coupled streptavidin followed by TMB substrate reaction. Shown are mean values ± SE of 3 individual experiments.