Construction and expression of eukaryotic plasmids containing lamivudine-resistant or wild-type strains of Hepatitis B Virus genotype C

Table 2 Real-time PCR detection of HBV DNA in transfected HepG2 cells (× 10⁸ copies/mL)

Clone	48 h	72 h
pcDNA3.1 (+)1	-	-
pcDNA3.1 (+)-HBV/C YMDD	3.57 ± 0.084	3.80 ± 0.078
pcDNA3.1 (+)-HBV/C YIDD	6.85 ± 0.143	6.90 ± 0.038
pcDNA3.1 (+)-HBV/C YVDD	17.64 ± 0.240	18.55 ± 0.127

The HBV DNA levels ≥ 5 × 10² copies/mL was considered as positive HBV DNA response.

¹The results were negative.