Replication of clinical hepatitis B virus isolate and its application for selecting antiviral agents for chronic hepatitis B patients

Table 2 Viral loads in supernatants of Huh7 cells transfected with HBV replicon (copies/mL)

Replicons	Transfected time (h)
	24	48	72	96
1	5.6 × 102	2.5 × 103	3.6 × 105	3.9 × 105
2	5.2 × 102	2.0 × 103	2.8 × 105	2.7 × 105
3	4.5 × 102	1.8 × 103	2.4 × 105	2.6 × 105
4	< 102	1.1 × 103	7.5 × 104	9.7 × 104
5	< 102	1.2 × 103	8.2 × 104	9.1 × 104
6	< 102	1.5 × 103	7.8 × 104	8.9 × 104
7	< 102	1.3 × 103	9.5 × 104	1.2 × 105
8	5.5 × 102	2.3 × 103	2.8 × 105	2.9 × 105
pHBV1.3	6.5 × 102	2.8 × 103	2.5 × 105	2.7 × 105
pHY106	< 102	< 102	< 102	< 102

pHBV1.3 is positive control; pHY106 is negative control.