Hepatic stellate cells may be potential effectors of platelet activating factor induced portal hypertension

Figure 3 A: Saturation curve of ³H-PAF binding to cultured cirrhotic hepatic stellate cells. ³H-PAF in the concentration between 0.125 and 3.2 nmol/L,in presence or the absence of 5 &mgr;mol/L unlabeled incubated at 25°C for 3 h; B: Scatchard plot analysis of binding of ³H-PAF to cirrhotic hepatic stellate cells. Cirrhosis: Kd: 4.66 nmol/L, Bax: 24.65 ± 1.96 fmol/&mgr;g DNA, R = 0.982; Control: Kd: 3.51 nmol/L, Bax: 5.74 ± 1.55 fmol/&mgr;g DNA, R = 0.93.