Transplanted bone marrow stromal cells are not cellular origin of hepatocellular carcinomas in a mouse model of carcinogenesis

Figure 3 Repopulation and carcinogenesis of male bone marrow-derived cells in female recipient liver tissues by FISH for Y chromosome (× 400). A: normal male liver, positive Y-chromosome signals appeared as green dots in the nuclei stained with DAPI, a chromosomal marker that appears as blue fluorescence; B: Six months after BMSCs transplantation and DEN treatment, some of hepatocyte nuclei were positive for the Y chromosome in the liver of female recipients; C: Six months after BMSCs transplantation without DEN treatment, none of hepatocyte nuclei was positive for Y chromosome in the liver of female recipients; D: HCC was negative for Y chromosome in nucleus.