Investigation of transcriptional gene silencing and mechanism induced by shRNAs targeted to RUNX3 in vitro

Figure 8 The cell cycle analysis by FCM. A: SGC790 cells, the cell proportions of G₀/G₁ and S stages were 43.2% ± 1.2% and 47.7% ± 1.1% in the SGC7901cell without transfection, respectively; B: pSilencer3.1-H1/SGC7901 cells, the cell proportions of G₀/G₁ and S stages were 40.3% ± 2.0% and 49.3% ± 0.9% in the SGC7901 cell with the plasmid DNA of pSilencer3.1-H1 respectively; C: pSilencer3.1-shRNA/RUNX3SGC7901 cells, the cell proportions of G₀/G₁ and S stages were 37.2% ± 1.9% and 60.5% ± 0.8% in the SGC790 cells transfected with the recombinated plasmid-pSilencer3.1-H1-shRNA/RUNX3 respectively. Compared with that of G₀/G₁ and S stages of two controls, the cell proportions of G₀/G₁ and S stages in the pSilencer3.1-H1-shRNA/RUNX3/SGC7901 cells decreased and increased obviously, respectively (P < 0.05).