Investigation of transcriptional gene silencing and mechanism induced by shRNAs targeted to RUNX3 in vitro

Figure 7 The colony formation assay of SGC7901 in the soft agar (×100). A: SGC7901 cells, the cloning efficiency was 9.9% ± 0.3% in the SGC7901 cell without transfection; B: pSilencer3.1-H1/SGC7901 cells, the cloning efficiency was 9.7% ± 0.6% in the SGC7901 cell with the plasmid DNA of pSilencer3.1-H1; C: pSilencer3.1-H1-shRNA/RUNX3/SGC 7901 cells, the cloning efficiency was 17.4% ± 0.31% in the SGC790 cells transfected with the recombinated plasmid-pSilencer3.1-H1-shRNA/RUNX3. A significant increase of the colony formation rate in the pSilencer3.1-H1-shRNA/RUNX3/SGC790 cells was discovered compared with the controls-the SGC-79011cells and pSilencer3.1-H1/SGC7901cells (P < 0.01).